AP09258993 Molecular evolution and ecology of influenza A viruses circulating among wild birds in Kazakhstan and identification of a new subtype
Publiction date: 08.09.2021 08:58
The current situation. Despite the successes achieved in studying the structure of the influenza virus, its genetic variability, ways of gene expression and replication and interaction with the host immune system, many questions of the evolution of the influenza virus, including the mechanisms of occurrence and persistence of epidemic and epizootic variants of viruses, remain unresolved. Their disclosure requires comparative virological and molecular biological studies of influenza A virus isolates circulating among wild birds in various regions of the world.
The existing problem. There is a need to collect biological materials from wild birds in the main migratory channels and various ecosystems of Kazakhstan, molecular screening and isolation of strains of influenza A viruses, genome-wide sequencing, as well as "metagenomic sequencing" by creating a library of genome samples, analyzing molecular markers that determine the range of hosts, studying variations in the diversity of nucleotide sequences of internal genes of isolates, their degrees of reassortment and interspecific transmission.
One of the important prerequisites for the development of the project was specific data on the circulation of a previously unidentified, new to science subtype of influenza A virus in the avifauna of the Republic of Kazakhstan, as well as new relevant and adapted to mammalian animals pathogenic and reassortant variants representing epidemic and epizootic significance.
Final results:
- for 2021: New isolates of influenza A viruses will be isolated from bioassays and HAA stored in the laboratory, and their antigenic formulas will be determined; Participation in a scientific conference will be taken.
- for 2022: The nucleotide composition of the genomes of influenza A viruses will be decoded by sequencing, their taxonomic affiliation will be established, and the amino acid sequences of the proteins encoded by them will be calculated;
- for 2023: Genetic diversity, the degree of reassortment and interspecific transmission in internal gene segments associated with different host species and subtypes on Kazakhstani isolates of avian influenza virus will be established. In accordance with the purpose and objectives of the project, at least 3 (three) will be published articles and (or) reviews in peer-reviewed scientific publications indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five); or at least 2 (two) articles and (or) reviews in peer-reviewed scientific publications indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five), and at least 1 (one) patent included in the Derwent Innovations Index database (Web of Science, Clarivate Analytics), as well as at least 1 (one) article or review in a peer-reviewed foreign or domestic publication recommended by COXON, or at least 2 (two) articles and (or) reviews in peer-reviewed scientific publications, included in the 1st (first) and (or) 2nd (second) quartiles in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 65 (sixty-five); or at least 1 (one) article or review in a peer-reviewed scientific publication included in the 1st (first) quartile in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 80 (eighty).
Description of direct results. The degree of prevalence of various subtypes of influenza A viruses among susceptible wild bird species will be established; a subtype of influenza A virus new to world science will be described and means for their identification in PCR will be developed; amino acid sequences of receptor-binding and proteolytic sites on will be determined and the structure of the RV2 protein for the presence of molecular determinants determining the range of hosts will be studied; Genetic diversity, the degree of reassortment and interspecific transmission in the internal gene segments of Kazakhstani avian influenza virus isolates associated with different host species and subtypes of NA will be established; Evolutionary trees will be constructed and the phylogeny of Kazakhstani avian influenza viruses will be determined; The breadth of origin of the sources of the avian influenza virus gene pool at the geographical point of collection of material will be characterized; In international databases the nucleotide sequences of the genes of new variants of the avian influenza virus will be deposited in the data; Optimal strains of avian influenza virus will be selected for the development and production of vaccine and diagnostic drugs.
Project Manager - Kydyrmanov Aydin Isagalievich, Doctor of Veterinary Sciences, Researcher ID: P-8825-2019; Scopus author ID: 6507024441, https://www.scopus.com/authid/detail.uri?authorId=6507024441
Performers:
Karamendin Kobei Omertaevich – Candidate of Veterinary Sciences,
Scopus author ID: 6505628190, https://www.scopus.com/authid/detail.uri?authorId=6505628190
Kassymbekov Ermukhammet Torebekovich, Master of Veterinary Medicine, Scopus author ID: 57193008573, https://www.scopus.com/authid/detail.uri?authorId=57193008573
Suleimenova Symbat Amangeldyevna, ORCID 0000-0003-4107-0681
Sabyrzhan Temirlan Berikuly, Master's student
The existing problem. There is a need to collect biological materials from wild birds in the main migratory channels and various ecosystems of Kazakhstan, molecular screening and isolation of strains of influenza A viruses, genome-wide sequencing, as well as "metagenomic sequencing" by creating a library of genome samples, analyzing molecular markers that determine the range of hosts, studying variations in the diversity of nucleotide sequences of internal genes of isolates, their degrees of reassortment and interspecific transmission.
One of the important prerequisites for the development of the project was specific data on the circulation of a previously unidentified, new to science subtype of influenza A virus in the avifauna of the Republic of Kazakhstan, as well as new relevant and adapted to mammalian animals pathogenic and reassortant variants representing epidemic and epizootic significance.
Final results:
- for 2021: New isolates of influenza A viruses will be isolated from bioassays and HAA stored in the laboratory, and their antigenic formulas will be determined; Participation in a scientific conference will be taken.
- for 2022: The nucleotide composition of the genomes of influenza A viruses will be decoded by sequencing, their taxonomic affiliation will be established, and the amino acid sequences of the proteins encoded by them will be calculated;
- for 2023: Genetic diversity, the degree of reassortment and interspecific transmission in internal gene segments associated with different host species and subtypes on Kazakhstani isolates of avian influenza virus will be established. In accordance with the purpose and objectives of the project, at least 3 (three) will be published articles and (or) reviews in peer-reviewed scientific publications indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five); or at least 2 (two) articles and (or) reviews in peer-reviewed scientific publications indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five), and at least 1 (one) patent included in the Derwent Innovations Index database (Web of Science, Clarivate Analytics), as well as at least 1 (one) article or review in a peer-reviewed foreign or domestic publication recommended by COXON, or at least 2 (two) articles and (or) reviews in peer-reviewed scientific publications, included in the 1st (first) and (or) 2nd (second) quartiles in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 65 (sixty-five); or at least 1 (one) article or review in a peer-reviewed scientific publication included in the 1st (first) quartile in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 80 (eighty).
Description of direct results. The degree of prevalence of various subtypes of influenza A viruses among susceptible wild bird species will be established; a subtype of influenza A virus new to world science will be described and means for their identification in PCR will be developed; amino acid sequences of receptor-binding and proteolytic sites on will be determined and the structure of the RV2 protein for the presence of molecular determinants determining the range of hosts will be studied; Genetic diversity, the degree of reassortment and interspecific transmission in the internal gene segments of Kazakhstani avian influenza virus isolates associated with different host species and subtypes of NA will be established; Evolutionary trees will be constructed and the phylogeny of Kazakhstani avian influenza viruses will be determined; The breadth of origin of the sources of the avian influenza virus gene pool at the geographical point of collection of material will be characterized; In international databases the nucleotide sequences of the genes of new variants of the avian influenza virus will be deposited in the data; Optimal strains of avian influenza virus will be selected for the development and production of vaccine and diagnostic drugs.
Project Manager - Kydyrmanov Aydin Isagalievich, Doctor of Veterinary Sciences, Researcher ID: P-8825-2019; Scopus author ID: 6507024441, https://www.scopus.com/authid/detail.uri?authorId=6507024441
Performers:
Karamendin Kobei Omertaevich – Candidate of Veterinary Sciences,
Scopus author ID: 6505628190, https://www.scopus.com/authid/detail.uri?authorId=6505628190
Kassymbekov Ermukhammet Torebekovich, Master of Veterinary Medicine, Scopus author ID: 57193008573, https://www.scopus.com/authid/detail.uri?authorId=57193008573
Suleimenova Symbat Amangeldyevna, ORCID 0000-0003-4107-0681
Sabyrzhan Temirlan Berikuly, Master's student
